Mouse Eotaxin Recombinant
$70.00 – $2,700.00
| accession | P48298 |
| Source | Optimized DNA sequence encoding Mouse Eotaxin/CCL11 mature chain sp|P48298|24-97 was expressed in Escherichia Coli. |
| Molecular weight | Mouse Eotaxin has a calculated molecular mass of approximately 8 kDa. Recombinant mouse Eotaxin is a monomer protein consisting 74 amino acid residue subunits,and migrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Activity was determined by the ability to chemoattract human peripheral blood eosinophils using a concentration range of.1-20.0 ng/ml. |
| Protein Sequence | MQSSTALLFL LLTVTSFTSQ VLAHPGSIPT SCCFIMTSKK IPNTLLKSYK RITNNRCTLK AIVFKTRLGK EICADPKKKW VQDATKHLDQ KLQTPKP |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant mouse Eotaxin was lyophilized from a.2 μm filtered PBS solution. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Chemotaxis |
| Biological Process | Inflammatory-response |
| Molecular function | Cytokine |
Methods
Expression and release of Gal-3 by BM-derived mouse Eos.
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Chemotaxis and intracellular calcium flux in WT and HVCN1-deficient eosinophils.
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ORMDL3-dependent eosinophil migration and CD48-mediated degranulation (a) Basal and eotaxin-1 (100 nM)-induced [Ca2+]i levels in eosinophils treated with Control-siRNA (a total of 598 cells) or ORMDL3-siRNA (a total of 870 cells) by digital videofluorescence imaging after loading with Fura-2 AM.
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