Mouse Eotaxin Recombinant

Mouse Eotaxin Recombinant


accession P48298

Source Optimized DNA sequence encoding Mouse Eotaxin/CCL11 mature chain sp|P48298|24-97 was expressed in Escherichia Coli.
Molecular weight Mouse Eotaxin has a calculated molecular mass of approximately 8 kDa. Recombinant mouse Eotaxin is a monomer protein consisting 74 amino acid residue subunits,and migrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Activity was determined by the ability to chemoattract human peripheral blood eosinophils using a concentration range of.1-20.0 ng/ml.

Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant mouse Eotaxin was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine


Expression and release of Gal-3 by BM-derived mouse Eos.

  • Gal-3 in culture supernatant of Eos incubated with eotaxin-1 (100 nM) or media alone for 30 min or 6 h by Western blot analysis followed by densitometry (Mean ± SEM).

Chemotaxis and intracellular calcium flux in WT and HVCN1-deficient eosinophils.

  • Transwell migration assay was performed to compare the in vitro migration of WT and HVCN1-deficient eosinophils subjected to mEotaxin-1 at the indicated concentrations.

ORMDL3-dependent eosinophil migration and CD48-mediated degranulation (a) Basal and eotaxin-1 (100 nM)-induced [Ca2+]i levels in eosinophils treated with Control-siRNA (a total of 598 cells) or ORMDL3-siRNA (a total of 870 cells) by digital videofluorescence imaging after loading with Fura-2 AM.

  • (b) Chemotaxis of untreated, Control-siRNA-treated and ORMDL3-siRNA-treated eosinophils in response to eotaxin-1 (100 nM) in vitro.