Mouse Eotaxin Recombinant
Categories: CCL chemokinesRecombinant Mouse Cytokines$70.00 – $2,700.00
Description
Accession
P48298
Source
Optimized DNA sequence encoding Mouse Eotaxin/CCL11 mature chain sp|P48298|24-97 was expressed in Escherichia Coli.
Molecular weight
Mouse Eotaxin has a calculated molecular mass of approximately 8 kDa. Recombinant mouse Eotaxin is a monomer protein consisting 74 amino acid residue subunits,and migrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Activity was determined by the ability to chemoattract human peripheral blood eosinophils using a concentration range of.1-20.0 ng/ml.
Protein Sequence
MQSSTALLFL LLTVTSFTSQ VLAHPGSIPT SCCFIMTSKK IPNTLLKSYK RITNNRCTLK AIVFKTRLGK EICADPKKKW VQDATKHLDQ KLQTPKP
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse Eotaxin was lyophilized from a.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Methods
Expression and release of Gal-3 by BM-derived mouse Eos.
- Gal-3 in culture supernatant of Eos incubated with eotaxin-1 (100 nM) or media alone for 30 min or 6 h by Western blot analysis followed by densitometry (Mean ± SEM).
Chemotaxis and intracellular calcium flux in WT and HVCN1-deficient eosinophils.
- Transwell migration assay was performed to compare the in vitro migration of WT and HVCN1-deficient eosinophils subjected to mEotaxin-1 at the indicated concentrations.
ORMDL3-dependent eosinophil migration and CD48-mediated degranulation (a) Basal and eotaxin-1 (100 nM)-induced [Ca2+]i levels in eosinophils treated with Control-siRNA (a total of 598 cells) or ORMDL3-siRNA (a total of 870 cells) by digital videofluorescence imaging after loading with Fura-2 AM.
- (b) Chemotaxis of untreated, Control-siRNA-treated and ORMDL3-siRNA-treated eosinophils in response to eotaxin-1 (100 nM) in vitro.
