| accession |
P27548 |
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|
| Source |
Optimized DNA sequence encodingMouseCD40L TNF domain was expressed in Escherichia Coli.
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| Molecular weight |
Native Mouse soluble CD40 Ligand is generated by the proteolytic processingof the full length transmembrane protein, the molecule has a calculated molecular mass of approximately16 kDa. Recombinant CD40 Ligand is a monomer protein consisting of149 amino acid residue subunits, and migrates as an approximately16 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
|
| Purity |
>95%, as determined by SDS-PAGE and HPLC
|
| Biological Activity |
The ED(50) was determined by its ability to induce TNF-alpha and MIP-1 alpha production by murine splenocytes. and was found to be in the range of.2 ng/ml.
|
| Protein Sequence |
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL DKVEEEVNLH EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL NKEEKKENSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN LVMLENGKQL TVKREGLYYV YTQVTFCSNR EPSSQRPFIV GLWLKPSSGS ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV TEASQVIHRV GFSSFGLLKL
|
| Endotoxin |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
|
| Presentation |
Recombinant CD40L was lyophilized from a.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
|
| Reconstitution |
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
|
| Storage |
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
|
| Usage |
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
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|
|
| Molecular function |
Cytokine |
