Mouse beta Nerve Growth Factor Recombinant
$70.00 – $2,700.00
SKU: RKP01139
Categories: Neurotrophic factors, Recombinant Mouse Cytokines
Tags: beta ngf, ngf, ngfb, P01139
accession | P01139 |
Source | Optimized DNA sequence encoding Mouse Nerve Growth Factor mature chain was expressed in CHO. |
Molecular weight | Native Mouse beta Nerve Growth Factor is generated by the proteolytic removal of the signal peptide and propeptide the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant Mouse beta Nerve Growth Factor is a homodimeric protein consisting of 2x120 amino acid residue subunits, and migrates as an approximately 26 kDa protein under non-reducing and 13kDa reducing conditions in SDS-PAGE. |
Purity | >95%, as determined by SDS-PAGE and HPLC |
Biological Activity | The ED(50) was determined by the determined by its ability to stimulate chick E9 DRG neurite outgrowth was found to be in the range of1.0 ng/ml. |
Protein Sequence | MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPTAPIA ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT LDLDFQAHGT IPFNRTHRSK RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant mouse NGF was lyophilized from a.2 μm filtered solution inmM sodium acetate, pH.5. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Interactor | P07174 |
Molecular function | Growth-factor |
Molecular function | Metalloenzyme-inhibitor |
Molecular function | Protease-inhibitor |
Methods
Cell culture
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