Mouse Angiopoietin-1 Recombinant

////Mouse Angiopoietin-1 Recombinant

Mouse Angiopoietin-1 Recombinant

$160.00$3,500.00


accession O08538


Source Optimized DNA sequence encoding mature chain of Mouse Angiopoietin-1 including an C-terminal His tagwas expressed in HEK293 cells.
Molecular weight RecombinantAngpt1is a protein consisting of484 amino acid residue subunits, due to glycosylation migrates as an approximately 70kDa protein on SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Mouse Angiopoietin-1 is lyophilized from 0.2 μm filtered 20mM SOdium Phosphate, pH7.5 ,200mM NaCl, 5% Trehalose.
Reconstitution A quick spin of the vial followed by gently mixing in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL. Avoid vigorous shaking or vortex
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, the protein can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months, addition of a carrier protein to your working aliquots (0.1% BSA or HSA) is recommended for long term storage Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor Q02763 TIE2_HUMAN
Biological Process Angiogenesis
Biological Process Differentiation
Molecular function Developmental-protein

Methods

Schematic illustration of the key findings Flow restriction upon occlusion of a main arterial route results in downstream ischaemia and increases the shear forces on the vessel wall of upstream, preformed collateral vessels, inducing the release of different cytokines and chemokines (black triangles).

recombinant ANG1
  • Subsequently, ANG1 (black circles) induces transcriptional downregulation of Phd2, activating the NF-κB pathway.