Description
Accession
Q60994
Source
Optimized DNA sequence encoding Mouse ADIPOQ mature chain including a C-terminal 6xHis tag was expressed in CHO. Recombinant Mouse Adiponectin encodes amino acid residues 18-247.
Molecular weight
Recombinant ADIPOQ migrates as an approximately 26 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
etermined by a cytotoxic assay using M1 cells. The ED50 for this effect is 4.0-6.0 μg/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
ADIPOQ was Lyophilized from PBS, pH 7.2.
Sequence
RGHHHHHHHH VTTTEELAPA LVPPPKGTCA GWMAGIPGHP GHNGTPGRDG RDGTPGEKGE KGDAGLLGPK GETGDVGMTG AEGPRGFPGT PGRKGEPGEA AYVYRSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFYCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSVLLHLEV GDQVWLQVYG DGDHNGLYAD NVNDSTFTGF LLYHDTN
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P46414
Interactor
Q9DCM2
Interactor
Interactor
Q96A54
Molecular function
