| accession | P61148 |
| Source | Optimized DNA sequence encoding Mouseacidic FGFmature chain was expressed in Escherichia Coli. |
| Molecular weight | Native Mouse aFGF, generated by the proteolytic removal of the signal peptide and propeptide, the moleculte has a calculated mass of kDa. Recombinant FGF acidicis a disulfide-linkedmonomer protein consisting of amino acidsandmigrates as an approximately16 kDa protein under reducing and non-reducing conditions. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED50 as determined by thedose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ ng/ml, corresponding to a specific activity of ≥ x units/mg. |
| Protein Sequence | MAEGEITTFA ALTERFNLPL GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESAGEVY IKGTETGQYL AMDTEGLLYG SQTPNEECLF LERLEENHYN TYTSKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant mouse aFGF was lyophilized from a.2 μm filtered PBS solution. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Biological Process | Angiogenesis |
| Biological Process | Differentiation |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
| Molecular function | Heparin-binding |
Methods
BMP and FGF are robust trophic factors for purified astrocytes.
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