Mouse acidic Fibroblast Growth Factor Recombinant

///Mouse acidic Fibroblast Growth Factor Recombinant

Mouse acidic Fibroblast Growth Factor Recombinant

$70.00$1,350.00

SKU: RKP61148 Category: Tags: , , , , ,

accession P61148


Source Optimized DNA sequence encoding Mouseacidic FGFmature chain was expressed in Escherichia Coli.
Molecular weight Native Mouse aFGF, generated by the proteolytic removal of the signal peptide and propeptide, the moleculte has a calculated mass of kDa. Recombinant FGF acidicis a monomer protein consisting of amino acidsandmigrates as an approximately16 kDa protein under reducing and non-reducing conditions.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED50 as determined by thedose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence MAEGEITTFA ALTERFNLPL GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESAGEVY IKGTETGQYL AMDTEGLLYG SQTPNEECLF LERLEENHYN TYTSKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant mouse aFGF was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Angiogenesis
Biological Process Differentiation
Molecular function Developmental-protein
Molecular function Growth-factor
Molecular function Heparin-binding

Methods

BMP and FGF are robust trophic factors for purified astrocytes.

10 ng/ml FGF1
  • Additional FGF family members were examined, but only FGF1 also had a modest trophic effect at 10 ng/ml .