Monoclonal mouse anti human TRAIL(APO2L)

///Monoclonal mouse anti human TRAIL(APO2L)

Monoclonal mouse anti human TRAIL(APO2L)

$99.00$149.00


accession P50591


Applications

ELISA:This antibody can be used at 2 μg/mLwith the appropriate secondary reagents to detect TRAIL/APO2L. The detection limit for recombinant human TRAIL is approximately 0.2 ng/well.

Source This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human TRAIL.
Species reactivity Human
Purification The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Presentation Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor Q9UBN6 TR10D_HUMAN
Interactor Q13158
Interactor Q14790 CASP8_HUMAN
Molecular function Cytokine

Methods

Expression of lytic molecules by IL-15 DCs.

TRAIL mAbs
  • Matured CD56+ IL-15 DCs were analyzed by flow cytometry for cell surface expression of TNF-α, FasL and TRAIL (solid line histograms).

Flow cytometric analysis of death receptor expression on the cell surface

  • The cell surface expression of the death receptors, TRAIL-R1 and TRAIL-R2, was determined by flow cytometry ( , Immunocytometry ).
  • LNCaP cells (2×105/ml) were seeded in 24-well plates for 24 h and exposed to artepillin C (50 and 100 μM) for 24 h. The cells were then harvested using a solution of trypsin and ethylenediaminetetra acetic acid (EDTA), washed twice in PBS and resuspended in PBS containing 0.5% bovine serum albumin (BSA).
  • LNCaP cells were incubated with 10 μl phycoerythrin-conjugated anti-TAIL-1 or anti-TAIL-2 monoclonal antibody at 4°C for 45 min.
  • After staining, the cells were washed with PBS and analyzed using flow cytometry (1 or mouse IgG2B .