/, Cytokines/Human TNF-alpha Recombinant (CHO cell )

Human TNF-alpha Recombinant (CHO cell )

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$380.00$3,050.00

SKU: RCP01375 Tags: , , , , , , , , , ,

Description

Accession
P01375
Source
Optimized DNA sequence encoding Human Tumor Necrosis Factor-alpha mature chain was expressed in CHO Cells.
Molecular weight
Native human Tumor Necrosis Factor-alpha is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 18 kDa. Recombinant TNF-alpha is a disulfide-linked trimeric protein,consisting of 157 amino acids and migrates as an approximately 18 kDa protein under reducing conditions.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determinedcytolysis of murine L929 cells in the presence of Actinomycin D is ≤.02 ng/ml, corresponding to a specific activity of >x units/mg.
Protein Sequence
MSTESMIRDV ELAEEALPKK TGGPQGSRRC LFLSLFSFLI VAGATTLFCL LHFGVIGPQR EEFPRDLSLI SPLAQAVRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
TNF-a was lyophilized from a.2μm filtered concentrated (1mg/ml) solution in PBS, pH.2.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O88351
Interactor
O88522
Interactor
O89110
Interactor
Interactor
Interactor
Interactor
P39429
Interactor
P62991
Interactor
Q12933
Interactor
Q15628
Interactor
Q60680
Interactor
Q60769
Interactor
Q60855
Interactor
Q62073
Interactor
Q924T7
Interactor
Q9QZL0
Interactor
Interactor
Interactor
Interactor
Interactor
P70191
Interactor
Q13546
Interactor
Q3U0V2
Interactor
Q80TQ2
Interactor
Q9DHW0
Interactor
Q9QZL0
Molecular function
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