///Anti SARS-COV2-S1 Monoclonal Antibody, CloneID 003

Anti SARS-COV2-S1 Monoclonal Antibody, CloneID 003

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$300.00$1,200.00

SKU: RMANCOVS13 Tags: , ,

Description

Accession
RKNCOVS1
Applications

ELISA: This antibody was validated by ELISA binding to recombinant SARS CoV2 RBD Fc (cat#RKNCOVRBD) with a EC50 binding affinity of 0.00447 and to the SARS CoV2 S1 (cat#RKNCOVS1H) with a binding affinity of 0.011(see images below).

Source
The recombinant antibody was produced in HEK293 cells. It is a chimera of a mouse variable chain, obtained from mice immunized with the SARS COV2 RBD protein ,fused to human IgG1.
CloneID
003
Species reactivity
Specific for SARS CoV2 S1 and the RBD domain, has not been tested against other viruses.
Isotype
Human IgG1 Kappa
Purification
The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
Presentation
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS with 5% trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
This antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Methods

Anti SARS COV-2-S1 monoclonal SDS PAGE

  • Reduced SDS PAGE of 10ug loaded sample

SARS COV-2-S1 ELISA

  • Recombinant SARS CoV2 S1-His protein (cat#RKNCOVS1H) at 100ng/well binding Anti S1 Monoclonal, with a calculated EC50 of 0.011

SARS COV-2 RBD ELISA

  • Recombinant SARS CoV2 RBD-Fc protein (cat#RMANCOVRBD) at 100ng/well binding Anti S1 Monoclonal, with a calculated EC50 of 0.00447
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