Monoclonal mouse anti human Interleukin-7

///Monoclonal mouse anti human Interleukin-7

Monoclonal mouse anti human Interleukin-7

$99.00$149.00

SKU: RMA13232 Category: Tags: , , ,

accession P13232


Applications

ELISA: This antibody can be used as capture antibody at 1-2 μg/mL jointly with biotinylated Anti IL7 polyclonal detection antibody to detect Human Interleukin-7. The detection limit for recombinant Human IL-7 is approximately 0.1 ng/well.

Western blot:This monoclonal antibody has been tested in WB analysis of human IL-7 . A suitable range of concentrations of this antibody for WB detection is 0.2-0.5 µg/ml.
The detection limit for Recombinant Human Interleukin-7 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.

Source This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human IL7.
Species reactivity Human IL-7.
Purification The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
Presentation Prepared from sterile filtered solution at a concentration of 1mg/ml in PBS with 5% trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage This antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P31785 IL2RG_HUMAN
Interactor P16871 IL7RA_HUMAN
Molecular function Cytokine
Molecular function Growth-factor