Monoclonal mouse anti human Interleukin-3

///Monoclonal mouse anti human Interleukin-3

Monoclonal mouse anti human Interleukin-3

$99.00$149.00

SKU: RMA08700 Category: Tags: , , , ,

accession P08700


Applications

ELISA: This antibody can be used as capture antibody at 2 μg/mL jointly with biotinylated Anti IL3 polyclonal detection antibody to detect Human Interleukin-3. The detection limit for recombinant Human IL-3 is approximately 0.1 ng/well.

Western blot:This monoclonal antibody has been tested in WB analysis of human IL-3 . A suitable range of concentrations of this antibody for WB detection is 1-2 µg/ml.
The detection limit for Recombinant Human Interleukin-3 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.

Source This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human IL3.
Species reactivity Human IL-3.
Purification The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
Presentation Prepared from sterile filtered solution at a concentration of 1mg/ml in PBS with 5% trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage This antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P26951 IL3RA_HUMAN
Interactor P32927 IL3RB_HUMAN
Molecular function Cytokine
Molecular function Growth-factor