ELISA:This antibody can be used at 2 μg/mL with the appropriate secondary reagents to detect Human IFN-gamma. The detection limit for Human IFN-gamma is approximately 0.2 ng/well.
|Source||This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human IFN-gamma.|
|Species reactivity||Human IFN-gamma|
|Purification||The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.|
|Presentation||Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS, pH 7.4.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers|
|Storage||The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|