Monoclonal Mouse anti Human BMP-7
$99.00 – $149.00
| accession | P18075 |
| Applications | Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-7 (50 ng/ml), a concentration of 0.2 - 0.6 µg/ml of this antibody is required. Western blot:This monoclonal antibody has been tested in WB analysis of human BMP-7 . A suitable range of concentrations of this antibody for WB detection is 2 µg/ml. With the appropriate secondary reagents the detection limit for recombinant BMP-7 is 3 ng/well.ELISA: This antibody can be used at 1μg/mL with the appropriate secondary reagents to detect BMP-7. The detection limit for recombinant BMP-7 is approximately 0.5 ng/well. |
| Source | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human BMP-7. |
| Species reactivity | Human BMP-7 |
| Purification | The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography. |
| Presentation | Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.2. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers |
| Storage | The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | Q13873 BMPR2_HUMAN |
| Interactor | P36898 BMR1B_MOUSE |
| Interactor | P37023 ACVL1_HUMAN |
| Biological Process | Chondrogenesis |
| Biological Process | Differentiation |
| Biological Process | Osteogenesis |
| Molecular function | Cytokine |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
