Monoclonal Mouse anti Human BMP-7
$99.00 – $149.00
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-7 (50 ng/ml), a concentration of 0.2 - 0.6 µg/ml of this antibody is required.
Western blot:This monoclonal antibody has been tested in WB analysis of human BMP-7 . A suitable range of concentrations of this antibody for WB detection is 2 µg/ml.With the appropriate secondary reagents the detection limit for recombinant BMP-7 is 3 ng/well.
ELISA: This antibody can be used at 1μg/mL with the appropriate secondary reagents to detect BMP-7. The detection limit for recombinant BMP-7 is approximately 0.5 ng/well.
|Source||This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human BMP-7.|
|Species reactivity||Human BMP-7|
|Purification||The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.|
|Presentation||Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.2.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers|
|Storage||The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|