Monoclonal Mouse anti Human BMP-4

///Monoclonal Mouse anti Human BMP-4

Monoclonal Mouse anti Human BMP-4

$99.00$149.00

SKU: RMA12644 Category: Tags: , , , , ,

accession P12644


Applications

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (2 ng/ml), a concentration of 2 - 4 µg/ml of this antibody is required.

Western blot:This monoclonal antibody has been tested in WB analysis of human BMP-4 . A suitable range of concentrations of this antibody for WB detection is 2 µg/ml.

With the appropriate secondary reagentsthe detection limit for Human BMP-4 is approximately1 ng/well.

ELISA: This antibody can be used at 1μg/mL with the appropriate secondary reagents to detect FGF-acidic. The detection limit for Human BMP-4 is approximately 0.5 ng/well.

Source This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human BMP-4.
Species reactivity Human BMP-4
Purification The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
Presentation Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.2.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O88273
Interactor P01730 CD4_HUMAN
Interactor P36895 BMR1A_MOUSE
Interactor Q13873 BMPR2_HUMAN
Interactor Q05438
Interactor Q62356 FSTL1_MOUSE
Biological Process Chondrogenesis
Biological Process Differentiation
Biological Process Osteogenesis
Molecular function Cytokine
Molecular function Developmental-protein
Molecular function Growth-factor

Methods

Western Blot

  • Ten µg of protein extract was loaded on acrylamide gel for SDS-PAGE electrophoresis.
  • Western blotting was carried out on nitrocellulose membrane blocked with PBS/0.05% Tween-20/2.5% BSA and probed with rabbit anti-BMP2 , mouse anti-BMP4 , goat anti-BMP5 , goat anti-BMP6 , rabbit anti-BMP7 overnight in PBS/0.05% Tween-20, 1% BSA.
  • Membranes were washed three times for 10 min each with PBS/0.05% Tween-20 and incubated with the appropriate secondary IgGs coupled to HRP.
  • Membranes were visualized using the ECL Plus detection kit on Biomax film .
  • To assess equal protein loading, membranes were stripped and re-probed with anti-GAPDH .