ELISA:This antibody can be used at 2 μg/ml with the appropriate secondary reagents to detect human NGF-beta. The detection limit for recombinant beta NGF is approximately 0.1 ng/well.
|Source||This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human Nerve Growth Factor.|
|Purification||The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.|
|Presentation||Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.4.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers|
|Storage||The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|