ELISA: This antibody can be used at 0.5-1μg/mLwith theappropriate secondaryreagents to detect FGF-acidic. The detection limit for FGF-acidic is approximately 2ng/well.
Western blot:This monoclonal antibody has been tested in WB analysis of human aFGF . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
|Source||This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human aFGF.|
|Species reactivity||Human aFGF|
|Purification||The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.|
|Presentation||Prepared from sterile filtered solution at a concentration of 1mg/ml in PBS with 5% trehalose.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers|
|Storage||This antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|
Monoclonal Mouse anti Human aFGF
$99.00 – $149.00