MERS Spike protein S1 Recombinant
$350.00 – $1,200.00
| accession | K0BRG7 |
| Source | DNA sequence encoding the extracellular domain of MERS-CoV Spike S1 including a C-terminal His tag was expressed in HEK293 Cells. |
| Molecular weight | Recombinant MERS-CoV Spike is a protein consisting of amino acid residues [Tyr 18-Glu725] ,due to glycosylation migrates as an approximately 117 kDa protein on SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The activity was determined by its ability to bind Human CD26 in functional ELISA assay with a linear range of 0.2-0.5ug/ml. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Spike was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Sequence | YVDVGPDSVK SACIEVDIQQ TFFDKTWPRP IDVSKADGII YPQGRTYSNI TITYQGLFPY QGDHGDMYVY SAGHATGTTP QKLFVANYSQ DVKQFANGFV VRIGAAANST GTVIISPSTS ATIRKIYPAF MLGSSVGNFS DGKMGRFFNH TLVLLPDGCG TLLRAFYCIL EPRSGNHCPA GNSYTSFATY HTPATDCSDG NYNRNASLNS FKEYFNLRNC TFMYTYNITE DEILEWFGIT QTAQGVHLFS SRYVDLYGGN MFQFATLPVY DTIKYYSIIP HSIRSIQSDR KAWAAFYVYK LQPLTFLLDF SVDGYIRRAI DCGFNDLSQL HCSYESFDVE SGVYSVSSFE AKPSGSVVEQ AEGVECDFSP LLSGTPPQVY NFKRLVFTNC NYNLTKLLSL FSVNDFTCSQ ISPAAIASNC YSSLILDYFS YPLSMKSDLS VSSAGPISQF NYKQSFSNPT CLILATVPHN LTTITKPLKY SYINKCSRLL SDDRTEVPQL VNANQYSPCV SIVPSTVWED GDYYRKQLSP LEGGGWLVAS GSTVAMTEQL QMGFGITVQY GTDTNSVCPK LEFANDTKIA SQLGNCVEYS LYGVSGRGVF QNCTAVGVRQ QRFVYDAYQN LVGYYSDDGN YYCLRACVSV PVSVIYDKET KTHATLFGSV ACEHISSTMS QYSRSTRSML KRRDSTYGPL QTPVGCVLGL |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P27487 DPP4_HUMAN |
