Human WNT3A Recombinant

Human WNT3A Recombinant

$70.00$4,200.00

SKU: RKP56704 Category: Tags: , ,

accession P56704


Source DNA sequence encoding Human WNT3A mature chain was expressed in HEK293 cells. Recombinant Wnt-3a encodes amino acid residues 19-352.
Molecular weight Recombinant human WNT3A migrates as an approximately 40 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 ​for this effect is 0.4-0.6 ng/ml.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation WNT3A was Lyophilized from PBS, pH 7.2.
Sequence SYPIWWSLAV GPQYSSLSTQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GVKAGIQECQ HQFRGRRWNC TTVSNSLAIF GPVLDKATRE SAFVHAIASA GVAFAVTRSC AEGSAAICGC SSRLQGSPGE GWKWGGCSED IEFGGMVSRE FADARENRPD ARSAMNRHNN EAGRQAIASH MHLKCKCHGL SGSCEVKTCW WSQPDFRTIG DFLKDKYDSA SEMVVEKHRE SRGWVETLRP RYTYFKVPTE RDLVYYEASP NFCEPNPETG SFGTRDRTCN VSSHGIDGCD LLCCGRGHNA RTERRREKCH CVFHWCCYVS CQECTRVYDV HTCK
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O75581
Interactor Q8J025 APCD1_HUMAN
Molecular function Developmental-protein