Human VEGFR2 Recombinant

//Human VEGFR2 Recombinant

Human VEGFR2 Recombinant


accession P35968

Source Optimized DNA sequence encoding mature chain of human Vascular endothelial growth factor receptor 2 (VEGFR2) (MET1-GLU764) including an C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant human VEGFR2 (FLK-1) is a protein consisting of 770 amino acid residue subunits,due to glycosylation migrates as an approximately 100kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Biological Activity The activity was tested by the ability to inhibit proliferation of HUVEC cells. The ED50 measured for this effect i 10-20 ng/ml
Presentation Recombinant human Vascular endothelial growth factor receptor 2(VEGFR2) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant VEGFR2 (FLK-1) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P15692 VEGFA_HUMAN
Interactor P01730 CD4_HUMAN
Interactor P09619 PGFRB_HUMAN
Interactor P49763 PLGF_HUMAN
Interactor P52584
Interactor P49767 VEGFC_HUMAN
Biological Process Angiogenesis
Biological Process Differentiation
Biological Process Host-virus-interaction
Molecular function Developmental-protein
Molecular function Transferase
Molecular function Receptor