Human VEGF 165AA Recombinant (CHO Cell)
$160.00 – $3,500.00
| accession | P15692 |
| Source | Optimized DNA sequence encoding HumanVEGF mature chain was expressed in CHO Cells |
| Molecular weight | Native humanVEGF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant Vascular endothelial growth factor is a homodimer protein consisting ofx165 amino acid residue subunits, and due to glycosylation migrates as an approximately kDa protein under non-reducing and askDa under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the dose-dependentproliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of.0-10.0 ng/ml. |
| Protein Sequence | MNFLLSWVHW SLALLLYLHH AKWSQAAPMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM SFLQHNKCEC RPKKDRARQE NPCGPCSERR KHLFVQDPQT CKCSCKNTDS RCKARQLELN ERTCRCDKPR R |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | RecombinantVEGF was lyophilized from.2 μm filteredmM PB solution, pH7.0 . |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | O60462 NRP2_HUMAN |
| Interactor | P07996 TSP1_HUMAN |
| Interactor | O14786 NRP1_HUMAN |
| Interactor | P01730 CD4_HUMAN |
| Interactor | P17948 VGFR1_HUMAN |
| Interactor | P35918 VGFR2_MOUSE |
| Interactor | P35968 VGFR2_HUMAN |
| Interactor | P49763 PLGF_HUMAN |
| Biological Process | Angiogenesis |
| Biological Process | Differentiation |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
| Molecular function | Heparin-binding |