Human VEGF 121AA Recombinant (E.Coli)

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Human VEGF 121AA Recombinant (E.Coli)

$70.00$3,500.00


accession P15692


Source Optimized DNA sequence encoding Human Vascular Endothelial Growth Factor mature chain was expressed in Escherichia Coli.
Molecular weight Native human VEGF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant Vascular endothelial growth factor is a disulfide-linked homodimer protein consisting of amino acid residue subunits, and migrates as an approximately kDa protein under non-reducing and askDa under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent proliferation ofhuman umbilical vein endothelial cells and was found to be in the range of.0-4.0 ng/ml.

Protein Sequence MNFLLSWVHW SLALLLYLHH AKWSQAAPMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM SFLQHNKCEC RPKKDRARQE NCDKPRR
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant VEGFwas lyophilized from.2 μm filtered PBS solution, pH.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O60462 NRP2_HUMAN
Interactor P07996 TSP1_HUMAN
Interactor O14786 NRP1_HUMAN
Interactor P01730 CD4_HUMAN
Interactor P17948 VGFR1_HUMAN
Interactor P35918 VGFR2_MOUSE
Interactor P35968 VGFR2_HUMAN
Interactor P49763 PLGF_HUMAN
Biological Process Angiogenesis
Biological Process Differentiation
Molecular function Developmental-protein
Molecular function Growth-factor
Molecular function Heparin-binding