Human Urokinase-type plasminogen activator Recombinant

////Human Urokinase-type plasminogen activator Recombinant

Human Urokinase-type plasminogen activator Recombinant


SKU: RKP00749 Category: Tags: , , ,

accession P00749

Source Optimized DNA sequence encoding peptidase domain of Human uPA (SER21 - LEU431) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant human Urokinase-type plasminogen activator (uPA) is a protein consisting of 420 amino acid residue subunits,due to glycosylation and cleavage migrates as an approximately 18kDa,32kDa,50kDa bands protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological activity The activity was tested by the ability of cleave the fluorogenic peptide substrate and was measured to be >2 nMoles/min/ug
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Furin is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant uPA can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor Q03405 UPAR_HUMAN
Biological Process Hemostasis
Biological Process Plasminogen-activation
Molecular function Hydrolase