Human Transcobalamin II Recombinant

//Human Transcobalamin II Recombinant

Human Transcobalamin II Recombinant


accession P20062

Source Optimized DNA sequence encoding mature chain of Human Transcobalamin II- TCII (GLU19 - TRP427 ) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant Human Transcobalamin II (TC2)is a protein consisting of 416 amino acid residue subunits,due to glycosylation migrates as an approximately as 47kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by the ability of immobilized Recombinant Human CD320 to bind recombinant human TCII .The ED50 was calculated to be < 1ng/ml
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant human Transcobalamin IIis lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant Transcobalamin II can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Transport