Human TNFSF10 Recombinant
$60.00 – $1,200.00
| accession | P50591 |
| Source | Optimized DNA sequence encoding Human TNFSF10 mature chain was expressed in ECOLI. Recombinant Human Tumor necrosis factor ligand superfamily member 10 encodes amino acid residues 1-281. |
| Molecular weight | Recombinant TNFSF10 migrates as an approximately 18 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | TNFSF10 was Lyophilized from PBS, pH 7.2. |
| Sequence | MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | Q9UBN6 TR10D_HUMAN |
| Interactor | Q13158 |
| Interactor | Q14790 CASP8_HUMAN |
| Molecular function | Cytokine |
