Description
Accession
O00220
Source
Optimized DNA sequence encoding Human TNFRSF10A mature chain was expressed in ECOLI. Recombinant Human Tumor necrosis factor receptor superfamily member 10A encodes amino acid residues 24-468.
Molecular weight
Recombinant TNFRSF10A migrates as an approximately 24 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Measured by its ability to inhibit apoptosis in LN-18 cells. The expected ED50 for this effect is 0.4 -0.5 µg/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
TNFRSF10A was Lyophilized from PBS, pH 7.2.
Sequence
SGTGAAAATP SKVWGSSAGR IEPRGGGRGA LPTSMGQHGP SARARAGRAP GPRPAREASP RLRVHKTFKF VVVGVLLQVV PSSAATIKLH DQSIGTQQWE HSPLGELCPP GSHRSERPGA CNRCTEGVGY TNASQQLFAC LPCTACKSDE EERSPCTTTR NTACQCKPGT FRNDNSAEMC RKCSTGCPRG MVKVKDCTPW SDIECVHKES GNGHN
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
