Human TNF-alpha Recombinant (CHO cell )

////Human TNF-alpha Recombinant (CHO cell )

Human TNF-alpha Recombinant (CHO cell )

$380.00$3,050.00


accession P01375


Source Optimized DNA sequence encoding Human Tumor Necrosis Factor-alpha mature chain was expressed in CHO Cells.
Molecular weight Nativehuman Tumor Necrosis Factor-alphais generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass ofapproximatelykDa. Recombinant TNF-alpha is a disulfide-linkedtrimeric protein,consisting of amino acidsandmigrates as an approximately18 kDa protein under reducing conditions.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determinedcytolysis of murine L929 cells in the presence of Actinomycin D is ≤.02 ng/ml, corresponding to a specific activity of >x units/mg.
Protein Sequence MSTESMIRDV ELAEEALPKK TGGPQGSRRC LFLSLFSFLI VAGATTLFCL LHFGVIGPQR EEFPRDLSLI SPLAQAVRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation TNF-a was lyophilized from a.2μm filtered concentrated (1mg/ml) solution in PBS, pH.2.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O88351
Interactor O88522
Interactor O89110
Interactor P01374 TNFB_HUMAN
Interactor P01730 CD4_HUMAN
Interactor P25118 TNR1A_MOUSE
Interactor P39429
Interactor P62991
Interactor Q12933
Interactor Q15628
Interactor Q60680
Interactor Q60769
Interactor Q60855
Interactor Q62073
Interactor Q924T7
Interactor Q9QZL0
Interactor P19438 TNR1A_HUMAN
Interactor P20333 TNR1B_HUMAN
Interactor P25118 TNR1A_MOUSE
Interactor P36941 TNR3_HUMAN
Interactor P70191
Interactor Q13546
Interactor Q3U0V2
Interactor Q80TQ2
Interactor Q9DHW0
Interactor Q9QZL0
Molecular function Cytokine