Human TNF-alpha Recombinant (CHO cell )
$380.00 – $3,050.00
| accession | P01375 |
| Source | Optimized DNA sequence encoding Human Tumor Necrosis Factor-alpha mature chain was expressed in CHO Cells. |
| Molecular weight | Native human Tumor Necrosis Factor-alpha is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 18 kDa. Recombinant TNF-alpha is a disulfide-linked trimeric protein,consisting of 157 amino acids and migrates as an approximately 18 kDa protein under reducing conditions. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determinedcytolysis of murine L929 cells in the presence of Actinomycin D is ≤.02 ng/ml, corresponding to a specific activity of >x units/mg. |
| Protein Sequence | MSTESMIRDV ELAEEALPKK TGGPQGSRRC LFLSLFSFLI VAGATTLFCL LHFGVIGPQR EEFPRDLSLI SPLAQAVRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | TNF-a was lyophilized from a.2μm filtered concentrated (1mg/ml) solution in PBS, pH.2. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | O88351 |
| Interactor | O88522 |
| Interactor | O89110 |
| Interactor | P01374 TNFB_HUMAN |
| Interactor | P01730 CD4_HUMAN |
| Interactor | P25118 TNR1A_MOUSE |
| Interactor | P39429 |
| Interactor | P62991 |
| Interactor | Q12933 |
| Interactor | Q15628 |
| Interactor | Q60680 |
| Interactor | Q60769 |
| Interactor | Q60855 |
| Interactor | Q62073 |
| Interactor | Q924T7 |
| Interactor | Q9QZL0 |
| Interactor | P19438 TNR1A_HUMAN |
| Interactor | P20333 TNR1B_HUMAN |
| Interactor | P25118 TNR1A_MOUSE |
| Interactor | P36941 TNR3_HUMAN |
| Interactor | P70191 |
| Interactor | Q13546 |
| Interactor | Q3U0V2 |
| Interactor | Q80TQ2 |
| Interactor | Q9DHW0 |
| Interactor | Q9QZL0 |
| Molecular function | Cytokine |