Human TLR3 Recombinant

Human TLR3 Recombinant

$60.00$2,500.00

SKU: RKO15455 Category: Tags: , ,

accession O15455


Source Optimized DNA sequence encoding Human TLR3 mature chain was expressed in CHO. Recombinant Human Toll-like receptor 3 encodes amino acid residues 24-904.
Molecular weight Recombinant TLR3 migrates as an approximately 75 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to neutralize Poly I:C induced NF-κB signaling in HT-29 cells stably transfected with NF-κB-luc. The ED50 for this effect is 5-10 µg/ml.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation TLR3 was Lyophilized from PBS, pH 7.2.
Sequence STTKCTVSHE VADCSHLKLT QVPDDLPTNI TVLNLTHNQL RRLPAANFTR YSQLTSLDVG FNTISKLEPE LCQKLPMLKV LNLQHNELSQ LSDKTFAFCT NLTELHLMSN SIQKIKNNPF VKQKNLITLD LSHNGLSSTK LGTQVQLENL QELLLSNNKI QALKSEELDI FANSSLKKLE LSSNQIKEFS PGCFHAIGRL FGLFLNNVQL GPSLTEKLCL ELANTSIRNL SLSNSQLSTT SNTTFLGLKW TNLTMLDLSY NNLNVVGNDS FAWLPQLEYF FLEYNNIQHL FSHSLHGLFN VRYLNLKRSF TKQSISLASL PKIDDFSFQW LKCLEHLNME DNDIPGIKSN MFTGLINLKY LSLSNSFTSL RTLTNETFVS LAHSPLHILN LTKNKISKIE SDAFSWLGHL EVLDLGLNEI GQELTGQEWR GLENIFEIYL SYNKYLQLTR NSFALVPSLQ RLMLRRVALK NVDSSPSPFQ PLRNLTILDL SNNNIANIND DMLEGLEKLE ILDLQHNNLA RLWKHANPGG PIYFLKGLSH LHILNLESNG FDEIPVEVFK DLFELKIIDL GLNNLNTLPA SVFNNQVSLK SLNLQKNLIT SVEKKVFGPA FRNLTELDMR FNPFDCTCES IAWFVNWINE THTNIPELSS HYLCNTPPHY HGFPVRLFDT SSCKDSAPFE L
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P01730 CD4_HUMAN
Biological Process Immunity
Biological Process Inflammatory-response
Molecular function Receptor
Molecular function RNA-binding