Human Tissue kallikrein Recombinant

//Human Tissue kallikrein Recombinant

Human Tissue kallikrein Recombinant


accession P06870

Source Optimized DNA sequence encoding Human Tissue kallikrein - KLK1 (PRO19-SER262) preprotein including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant Human Kallikrein-11 (KLK11) is a protein consisting of 250 amino acid residue subunits,due to glycosylation migrates as an approximately as 40kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by the ability to cleave a fluorogenic substrate PFR-AMC .The specific activity was measured to be 1.2 nmole/ug/min
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Tissue kallikrein is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant Tissue kallikrein (KLK1) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Molecular function Hydrolase