Human Tissue factor pathway inhibitor 2 Recombinant

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Human Tissue factor pathway inhibitor 2 Recombinant


accession P48307

Source Optimized DNA sequence encoding mature chain of human Tissue factor pathway inhibitor 2-TFPI2 (ASP23 - LYS213) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant human TFPI-2 is a protein consisting of 198 amino acid residue subunits,due to glycosylation migrates as an approximately 30kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological activity The activity was tested by the ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE- Nval-WRKDnp-NH2, with a calculated ED50 < 2.5nM
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Tissue factor pathway inhibitor 2 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant Tissue factor pathway inhibitor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Hemostasis
Molecular function Protease-inhibitor