/, IL-2 family, Recombinant Human Cytokines/Human Thymic stromal lymphopoietin Recombinant

Human Thymic stromal lymphopoietin Recombinant

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$70.00$4,700.00

SKU: RKQ969D9 Tags: ,

Description

Accession
Q969D9
Source
DNA sequence encoding Human TSLP mature chain was expressed in HEK293 cells
Molecular weight
Recombinant human TSLP is a protein consisting of Tyr29-Gln159 amino acid residue subunits, due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE
Purity
>95%, as determined by SDS-PAGE and HPLCThe activity as determined by binding Human TSLPR in an ELISA assay.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Human TSLP was Lyophilized from a.2μm filtered concentrated (1.0mg/ml) solution inmM PB, pH.4,mM NaCl.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function

Methods

Cytokine responses in function of CD25 expression.

  • Examples of pSTAT5 responses to IL-7, IL-2, and TSLP stimulation are shown in the CD4+ CD45RA- T cell population for one healthy donor (HD-15) and two ICL patients (ICL-P3 and ICL-P12).

IL-3 and TSLP mediate Eo/B differentiation from PB HHP.

  • (b) A representative overlay histogram for expression of IL-3Rα on unstimulated (shaded gray), IL-3 (dotted line), TSLP (gray line), and IL-3/TSLP (black line) stimulated CD34+ cells.
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