Human TGF Beta-2 Recombinant (CHO Cell)
$70.00 – $3,500.00
| accession | P01137 |
| Source | Optimized DNA sequence encoding Human Transforming Growth Factor Beta-2 mature chain was expressed in CHO Cells |
| Molecular weight | Native human TGF-b2, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately kDa. RecombinantTransforming Growth Factor Beta-2is a homodimer protein consisting ofx112 amino acid residue subunits. TGF-beta migrates as an approximately - kDa protein under reducing conditions in SDS-PAGE and askDa band under non reducing conditions. |
| Purity | >95%, as determined by SDS-PAGE and HPLC. |
| Protein Sequence | MPPSGLRLLL LLLPLLWLLV LTPGRPAAGL STCKTIDMEL VKRKRIEAIR GQILSKLRLA SPPSQGEVPP GPLPEAVLAL YNSTRDRVAG ESAEPEPEPE ADYYAKEVTR VLMVETHNEI YDKFKQSTHS IYMFFNTSEL REAVPEPVLL SRAELRLLRL KLKVEQHVEL YQKYSNNSWR YLSNRLLAPS DSPEWLSFDV TGVVRQWLSR GGEIEGFRLS AHCSCDSRDN TLQVDINGFT TGRRGDLATI HGMNRPFLLL MATPLERAQH LQSSRHRRAL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQA LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
| Presentation | Recombinant TGF-b2 is lyophilized from.2 μm filtered PBS solution pH7.4 . |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P01730 CD4_HUMAN |
| Interactor | P37023 ACVL1_HUMAN |
| Interactor | P18564 |
| Interactor | P36897 TGFR1_HUMAN |
| Interactor | P37173 TGFR2_HUMAN |
| Interactor | Q03167 |
| Interactor | Q14392 LRC32_HUMAN |
| Interactor | Q14766 |
| Interactor | Q62356 FSTL1_MOUSE |
| Molecular function | Growth-factor |
| Molecular function | Mitogen |
Methods
Treatment of microglia with IL4 increased TGFβ2 expression and secretion.
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Influence of TGFβ and TGFβ inhibitors on morphology and proliferation of NHU cell cultures.
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Collagen I and fibronectin protein expressions were not induced in SPARC knockdown HTFs in response to TGF-β2 but α-SMA expression remained inducible.
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Effects of Myo/Nog cell depletion and TGF-β2 on wound healing in anterior lens explants.
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