Human TGF Beta-2 Recombinant (CHO Cell)

////Human TGF Beta-2 Recombinant (CHO Cell)

Human TGF Beta-2 Recombinant (CHO Cell)

$70.00$3,500.00

SKU: RKP61812 Category: Tags: ,

accession P01137


Source Optimized DNA sequence encoding Human Transforming Growth Factor Beta-2 mature chain was expressed in CHO Cells
Molecular weight Native human TGF-b2, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately kDa. RecombinantTransforming Growth Factor Beta-2is a homodimer protein consisting ofx112 amino acid residue subunits. TGF-beta migrates as an approximately - kDa protein under reducing conditions in SDS-PAGE and askDa band under non reducing conditions.
Purity >95%, as determined by SDS-PAGE and HPLC.
Protein Sequence MPPSGLRLLL LLLPLLWLLV LTPGRPAAGL STCKTIDMEL VKRKRIEAIR GQILSKLRLA SPPSQGEVPP GPLPEAVLAL YNSTRDRVAG ESAEPEPEPE ADYYAKEVTR VLMVETHNEI YDKFKQSTHS IYMFFNTSEL REAVPEPVLL SRAELRLLRL KLKVEQHVEL YQKYSNNSWR YLSNRLLAPS DSPEWLSFDV TGVVRQWLSR GGEIEGFRLS AHCSCDSRDN TLQVDINGFT TGRRGDLATI HGMNRPFLLL MATPLERAQH LQSSRHRRAL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQA LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant TGF-b2 is lyophilized from.2 μm filtered PBS solution pH7.4 .
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P01730 CD4_HUMAN
Interactor P37023 ACVL1_HUMAN
Interactor P18564
Interactor P36897 TGFR1_HUMAN
Interactor P37173 TGFR2_HUMAN
Interactor Q03167
Interactor Q14392 LRC32_HUMAN
Interactor Q14766
Interactor Q62356 FSTL1_MOUSE
Molecular function Growth-factor
Molecular function Mitogen

Methods

Treatment of microglia with IL4 increased TGFβ2 expression and secretion.

recombinant human TGFβ2
  • Quantitative RT-PCR for TGFβ1 , TGFβ2 and TGFβ3 revealed increased TGFβ2 expression after IL4 treatment.

Influence of TGFβ and TGFβ inhibitors on morphology and proliferation of NHU cell cultures.

TGFβ2
  • (b) MTT assay showing dose-response curves of viable biomass from non-differentiated (proliferative) NHU cells treated for 4 days with TGFβ1 and TGFβ2.

Collagen I and fibronectin protein expressions were not induced in SPARC knockdown HTFs in response to TGF-β2 but α-SMA expression remained inducible.

recombinant TGF-β2
  • HTFs were treated with MMC (left panel) or transfected with siRNAs (right panel), with or without TGF-β2, for 72 hrs before being analysed for collagen I protein abundance by immunoblotting for collagen Iα1 and GAPDH (loading control).

Effects of Myo/Nog cell depletion and TGF-β2 on wound healing in anterior lens explants.

10 ηg/ml human recombinant TGF-β2
  • The wound was also covered with cells following treatment with TGF-β2 .