Human TGF Beta-1 Recombinant, Fc

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Human TGF Beta-1 Recombinant, Fc


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SKU: RKP01137F Categories: , Tags: , , , ,

accession P01137

Source Optimized DNA sequence encoding Human Transforming Growth Factor Beta-1 mature chain was expressed in HEK293 cells fused to a C terminal Human IgG1 Fc tag
Molecular weight . Recombinant TGF-beta 1 is a protein consisting of 374 amino acid residue subunits. TGF-beta 1 1 has a predicted MW of 42kDa.
Purity >95%, as determined by SDS-PAGE and HPLC.
Biological activity The activity was determined by the ability to inhibit the proliferation of mouse IL-4 dependent mouse HT-2 cells, with an ED50 of 0.1ng/ml corresponding to a specific activity of 1x 10^7 units/mg.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant TGF-b1 is lyophilized from 0.2 μm filtered 100mM glycine PH 3.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P01730 CD4_HUMAN
Interactor P37023 ACVL1_HUMAN
Interactor P18564
Interactor P36897 TGFR1_HUMAN
Interactor P37173 TGFR2_HUMAN
Interactor Q03167
Interactor Q14392 LRC32_HUMAN
Interactor Q14766
Interactor Q62356 FSTL1_MOUSE
Molecular function Growth-factor
Molecular function Mitogen