Human TECK (CCL25 ) Recombinant

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Human TECK (CCL25 ) Recombinant

$70.00$2,700.00


accession O15444


Source Optimized DNA sequence encoding Human CCL25 mature chain was expressed in Escherichia Coli.
Molecular weight Native human CCL25 has a calculated molecular mass of approximately 14 kDa. Recombinant CCL25 is a monomer protein consisting of 128 amino acid residue subunits, and migrates as an approximately 14 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity Activity was determined by the ability to induce chemotaxis of purified human monocytes at concentrations ranging between-10 ng/ml.

Protein Sequence MNLWLLACLV AGFLGAWAPA VHTQGVFEDC CLAYHYPIGW AVLRRAWTYR IQEVSGSCNL PAAIFYLPKR HRKVCGNPKS REVQRAMKLL DARNKVFAKL HHNTQTFQAG PHAVKKLSSG NSKLSSSKFS NPISSSKRNV SLLISANSGL
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant CCL25 was lyophilized from PBS pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O43927 CXL13_HUMAN
Interactor P51686
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

Serum CCL25 levels in LuCa patients Serum CCL25 levels in healthy donors (n=9), SCC (n=17) and AC (n=14) patients were analyzed by ELISA.

CCL25
  • As used, the ELISA could detect >5 pg/mL of CCL25.