Human Stem Cell Factor Recombinant Biotinylated

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Human Stem Cell Factor Recombinant Biotinylated


accession P21583

Source Optimized DNA sequence encoding Human SCF mature chain fused to PolyHis AVI tag was expressed in HEK293 Cells,was further labeled by Biotin for detection by Avidin conjugated secondary probes.
Molecular weight Native human Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18kDa. Recombinant SCF is a protein consisting of 165 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein under reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity Activity was tested by the ability to bind Human SCF receptor in a functional ELISA assay with a linear range of 0.1-5.0 ng/ml.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant SCF was lyophilized from a.2 μm filtered solution inPBSpH7.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Cell-adhesion
Molecular function Growth-factor