Human SPP1 Recombinant
$60.00 – $1,200.00
| accession | P10451 |
| Source | Optimized DNA sequence encoding Human SPP1 mature chain was expressed in ECOLI. Recombinant Human Osteopontin encodes amino acid residues 17-314. |
| Molecular weight | Recombinant SPP1 migrates as an approximately 33 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | SPP1 was Lyophilized from PBS, pH 7.2. |
| Sequence | IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P42574 CASP3_HUMAN |
| Interactor | Q14790 CASP8_HUMAN |
| Biological Process | Biomineralization |
| Biological Process | Cell-adhesion |
| Molecular function | Cytokine |
