Human SPAM1 (PH-20) Recombinant

//Human SPAM1 (PH-20) Recombinant

Human SPAM1 (PH-20) Recombinant


accession P38567

Source Optimized DNA sequence encoding extracellular domain of human SPAM1 (PH20) (LEU36 - TYR482 ) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant humanSPAM1 (PH20)is a protein consisting of 452 amino acid residue subunits,due to glycosylation migrates as an approximately as 64kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human SPAM-1 (PH-20) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage RecombinantSPAM-1 (PH-20)can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Cell-adhesion
Molecular function Hydrolase