Human SDF-1 alpha Recombinant, Biotinylated

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Human SDF-1 alpha Recombinant, Biotinylated


SKU: RBP48061 Categories: , Tags: , , , , , , , , , , ,

accession P48061

Source Optimized DNA sequence encoding Human Stromal Cell-Derived Factor-1 alpha mature chain was expressed in Escherichia Coli. SDF-1A was further labeled by Biotin for detection by Avidin conjugated secondary probes
Molecular weight Native human SDF-1 alpha, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately8 kDa. Recombinant SDF-1 alpha is a monomer protein consisting of69 amino acid residue subunits, migrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Applications ELISA,WB Biotinylated SDF-1A was designed to be used in single step detection in ELISA ,WB
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant SDF-1 alpha was lyophilized from a.2μm filtered PBS, pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P15172
Biological Process Chemotaxis
Molecular function Cytokine
Molecular function Growth-factor