Human SDF-1 alpha Recombinant, Biotinylated
$160.00 – $3,500.00
accession | P48061 |
Source | Optimized DNA sequence encoding Human Stromal Cell-Derived Factor-1 alpha mature chain was expressed in Escherichia Coli. SDF-1A was further labeled by Biotin for detection by Avidin conjugated secondary probes |
Molecular weight | Native human SDF-1 alpha, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately8 kDa. Recombinant SDF-1 alpha is a monomer protein consisting of69 amino acid residue subunits, migrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
Purity | >97%, as determined by SDS-PAGE and HPLC |
Applications | ELISA,WB Biotinylated SDF-1A was designed to be used in single step detection in ELISA ,WB |
Protein Sequence | MNAKVVVVLV LVLTALCLSD GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNK R FKM |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant SDF-1 alpha was lyophilized from a.2μm filtered PBS, pH.4. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Interactor | P15172 |
Biological Process | Chemotaxis |
Molecular function | Cytokine |
Molecular function | Growth-factor |