Human RANTES-CCL5 Recombinant
Categories: CCL chemokinesRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
P13501
Source
Optimized DNA sequence encoding Human Rantes/CCL5 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human RANTES/CCL5 isgenerated by the proteolytic removal of the signal peptideand propeptide, the molecule has a calculated molecular mass of approximately8 kDa. Recombinant RANTES/CCL5is a monomer protein consisting of amino acid residue subunits andmigrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human blood monocytes using a concentration range of.0-10.0 ng/ml.
Protein Sequence
MKVSAAALAV ILIATALCAP ASASPYSSDT TPCCFAYIAR PLPRAHIKEY FYTSGKCSNP AVVFVTRKNR QVCANPEKKW VREYINSLEM S
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Rantes/CCL5was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
P15172
Interactor
P32246
Interactor
P51681
Interactor
P32302
Interactor
P49682
Interactor
P51677
Biological Process
Biological Process
Molecular function
Methods
CCL5/RANTES or CX3CL1/Fractalkine binding to US28 triggers calcium release in HCMV infected smooth muscle but not glioblastoma cells.
- At 48 hpi, cells were labeled with Fluo-4 AM and stimulated with 10 nM CCL5/RANTES (top panels) or 10 nM CX3CL1/Fractalkine (bottom panels).
GLP-1(9-36) inhibits chemokine-induced migration of isolated human CD4-positive lymphocytes.
- A. and B.: Cells were pretreated with GLP-1(9-36) for 30 minutes at concentrations indicated before migration experiments using SDF-1 (100 ng/mL) or RANTES (100 ng/mL) were performed for three hours in the modified Boyden chamber.
Effects of CoCl2, an NF-κB inhibitor, and HO-1 RNA interference on TNF-α/IFN-γ-induced cytokine production.
- The production of RANTES and MCP-1 was analyzed in the supernatants of HK-2 cells using ELISA.
Interaction of CXCL447–70 and CXCL4L147–70 with CCL5 Changes in the expression of murine (mu) CCL5 within the tumor microenvironment were evaluated in vehicle (CO)-, CXCL447–70- and CXCL4L147–70-treated MDA-MB-231 tumors by qPCR (A).
- In order to further evaluate a possible interaction between CCL5 and the administered peptides, binding of biotinylated CXCL447–70 or CXCL4L147–70 to human CCL5-coated plates was quantified , whereas functional synergy was investigated by evaluating chemotaxis of monocytic cells .
