Human RANTES-CCL5 Recombinant , biotinylated

/, Recombinant Human Cytokines, CCL chemokines/Human RANTES-CCL5 Recombinant , biotinylated

Human RANTES-CCL5 Recombinant , biotinylated


accession P13501

Source DNA sequence encoding Human MIP-1A mature chain was expressed in Escherichia Coli. CCL5 was further labeled by Biotin for detection by Avidin conjugated secondary probes
Molecular weight Native human RANTES/CCL5 isgenerated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately8 kDa. Recombinant RANTES/CCL5is a monomer protein consisting of 68 amino acid residue subunits and migrates as an approximately8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Applications Biotinylated CCl5 was designed to be used in single step detection in ELISA ,WB
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Rantes/CCL5was lyophilized from a.2 μm filtered PBS solution.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P01730 CD4_HUMAN
Interactor P15172
Interactor P32246
Interactor P51681
Interactor P32302
Interactor P49682
Interactor P51677
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine