Human Prolactin Recombinant
$70.00 – $1,350.00
accession | P01236 |
Source | Optimized DNA sequence encoding HumanProlactin mature chain was expressed in Escherichia Coli. |
Molecular weight | Human Prolactin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately23 kDa. Recombinant Prolcatin is a monomeric protein consisting of200 amino acid residue subunits, and migrates as an approximately23 kDa protein under non-reducing and reducing conditions in SDS-PAGE. |
Purity | >98%, as determined by SDS-PAGE and HPLC |
Biological Activity | The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkeyMBr-5 cells was found to be in the range of.0-40.0 ng/ml. |
Protein Sequence | MNIKGSPWKG SLLLLLVSNL LLCQSVAPLP ICPGGAARCQ VTLRDLFDRA VVLSHYIHNL SSEMFSEFDK RYTHGRGFIT KAINSCHTSS LATPEDKEQA QQMNQKDFLS LIVSILRSWN EPLYHLVTEV RGMQEAPEAI LSKAVEIEEQ TKRLLEGMEL IVSQVHPETK ENEIYPVWSG LPSLQMADEE SRLSAYYNLL HCLRRDSHKI DNYLKLLKCR IIHNNNC |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant human Prolactin was lyophilized from a.2 μm filtered NaHCO3 solution. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Interactor | P16471 PRLR_HUMAN |
Interactor | Q13415 |
Biological Process | Lactation |
Molecular function | Hormone |
Methods
In Vitro Differentiation
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