Human PLGF1 Recombinant
$70.00 – $2,500.00
| accession | P49763 |
| Source | Optimized DNA sequence encoding Human Placenta growth factor isoform 1 mature chain was expressed in E.coli |
| Molecular weight | Native human Placenta growth factor (PLGF) is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 15 kDa. Recombinant PLGF is a homodimer protein consisting of 2x132 amino acid residue subunits, migrates as an approximately 30kDa protein under non reducing conditions in SDS-PAGE. |
| Purity | >97%, as determined by SDS-PAGE and HPLC |
| Biological Activity | The ED(50) was determined by the dose-dependent stimulation of the proliferation of human MDA cells is ≤ 10ug/ml. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant PLGF was lyophilized from a 0.2 μm filtered solution inPBSpH 7.0. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P35916 VGFR3_HUMAN |
| Interactor | P35968 VGFR2_HUMAN |
| Interactor | P01730 CD4_HUMAN |
| Interactor | P15692 VEGFA_HUMAN |
| Interactor | P17948 VGFR1_HUMAN |
| Biological Process | Angiogenesis |
| Biological Process | Differentiation |
| Molecular function | Developmental-protein |
| Molecular function | Growth-factor |
| Molecular function | Heparin-binding |