Human PLGF Recombinant (CHO Cell)

////Human PLGF Recombinant (CHO Cell)

Human PLGF Recombinant (CHO Cell)


accession P21583

Source Optimized DNA sequence encoding HumanPlacenta growth factor isoform 1 mature chain fused to a C terminal Fc tag was expressed in CHO Cells
Molecular weight Native human Placenta growth factor (PLGF) is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20 kDa. Recombinant PLGF is a protein consisting of 156 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein underreducing conditions in SDS-PAGE.
Purity >97%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent stimulation of theproliferation of human MDA cells is ≤ 10ug/ml.

Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant PLGF was lyophilized from a 0.2 μm filtered solution inPBSpH 7.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Cell-adhesion
Molecular function Growth-factor