Human PF4 (CXCL4) Recombinant

///Human PF4 (CXCL4) Recombinant

Human PF4 (CXCL4) Recombinant

$70.00$2,700.00


accession P02776


Source Optimized DNA sequence encoding Human PF4 CXCL4 mature chain was expressed in Escherichia Coli.
Molecular weight Native human PF4 is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8kDa. Recombinant PF4 is a monomer protein consisting of 70 amino acid residue subunits, and migrates as an approximately 8kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically-15 μg/mL.
Protein Sequence MSSAAGFCAS RPGLLFLGLL LLPLVVAFAS AEAEEDGDLQ CLCVKTTSQV RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIIKKLLE S
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant PF4/CXCL4 was lyophilized from a.2μm filtered concentrated (1mg/ml) solution inmM PB,1.5M NaCl pH.4.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Chemotaxis
Molecular function Cytokine
Molecular function Heparin-binding

Methods

PF4, collagen type I and IP-10 present in PEA material.

400 ng/ml PF4
  • Immunohistochemical stainings for PF4 , trichrome staining and IP-10 .

PF4, collagen type I and IP-10 present in PEA material.

1000 ng/ml PF4
  • Immunohistochemical stainings for PF4 , trichrome staining and IP-10 .