Human PD-L1 (B7-H1) Recombinant, Biotinylated
Category: Biotinylated proteins$340.00 – $1,950.00
Description
Accession
Q9NZQ7
Source
Optimized DNA sequence encoding extracellular domain of Human Programmed Death Ligand 1 ,PD-L1, (PHE19 - ARG238) including a C-terminal His tag was expressed in HEK293 cells. Recombinant Human PD-L1 was further labeled by Biotin for detection by Avidin conjugated secondary probes.
Molecular weight
Recombinant human PD-L1 (B7-H1) is a protein consisting of 225 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Applications
ELISA,WB Biotinylated recombinant PD-L1 was designed to be used in single step detection in ELISA ,WB
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human PD-L1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Storage
Recombinant B7-H1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
