Human PD-1 (PDCD1) Recombinant, Biotinylated

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Human PD-1 (PDCD1) Recombinant, Biotinylated

$340.00$1,950.00


accession Q15116


Source Optimized DNA sequence encoding extracellular domain of Human Programmed cell death protein 1 PD-1 (PRO21 - GLN167) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant human PD-1 (PDCD1) is a protein consisting of 150 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The activity was tested by the ability of immobilized recombinant Human PD-1 to bind recombinant human PD-L1 within a linear range of 0.1-5.0 ng/ml in ELISA.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human PD-1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in sterile PBS pH 7.4 to a concentration not less than 0.2 mg/mL. Solubilize for 1hr at room temp with gentle mixing.
Storage Recombinant PD-1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months.It is recommended to add 0.1% HSA or BSA for long term storage. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biological Process Immunity

Methods

Functional ELISA

  • 96-well maxisorp plates were coated at 1ug/well recombinant human PD-L1
  • Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.
  • Absorbance was measured at 450-615 nm using an automated plate reader.