Human Oncostatin M Recombinant
$70.00 – $4,700.00
accession | P13725 |
Source | Optimized DNA sequence encoding Human Oncostatin-M mature chain was expressed in Escherichia Coli. |
Molecular weight | Native human Oncostatin-M is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 26 kDa. Recombinant Oncostatin-M is a monomer protein consisting of 228 amino acid residue subunits, and migrates as an approximately 26 kDa protein under reducing conditions in SDS-PAGE. |
Purity | >95%, as determined by SDS-PAGE and HPLC |
Biological Activity | The ED(50) was determined by the dose-dependent stimulation of the proliferation off human TF-1 cells is ≤3 ng/ml, corresponding to a specific activity of ≥2 x units/mg. |
Protein Sequence | MGVLLTQRTL LSLVLALLFP SMASMAAIGS CSKEYRVLLG QLQKQTDLMQ DTSRLLDPYI RIQGLDVPKL REHCRERPGA FPSEETLRGL GRRGFLQTLN ATLGCVLHRL ADLEQRLPKA QDLERSGLNI EDLEKLQMAR PNILGLRNNI YCMAQLLDNS DTAEPTKAGR GASQPPTPTP ASDAFQRKLE GCRFLHGYHR FMHSVGRVFS KWGESPNRSR RHSPHQALRK GVRRTRPSRK GKRLMTRGQL PR |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg). |
Presentation | Recombinant Oncostatin M was lyophilized from a.2μm filtered concentrated (1mg/ml) PBS solution, pH7.0. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Interactor | P05231 IL6_HUMAN |
Interactor | P42702 LIFR_HUMAN |
Interactor | P40189 IL6RB_HUMAN |
Biological Process | Growth-regulation |
Molecular function | Cytokine |
Molecular function | Mitogen |
Methods
Hepatogenic differentiation
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Induction of mature hepatocytic functional genes by spheroid formation
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