Human Oncostatin M Recombinant (CHO Cell)

////Human Oncostatin M Recombinant (CHO Cell)

Human Oncostatin M Recombinant (CHO Cell)

$160.00$4,700.00

SKU: RCP13725 Category: Tags: , ,

accession P13725


Source Optimized DNA sequence encoding Human Oncostatin-M mature chain was expressed in CHO Cells
Molecular weight Native human Oncostatin-M is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 25 kDa. Recombinant Oncostatin-M is a protein consisting of 227 amino acid residue subunits, due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity The ED(50) was determined by the dose-dependent stimulation of the proliferation off human TF-1 cells is ≤3 ng/ml, corresponding to a specific activity of ≥2 x units/mg.
Protein Sequence MGVLLTQRTL LSLVLALLFP SMASMAAIGS CSKEYRVLLG QLQKQTDLMQ DTSRLLDPYI RIQGLDVPKL REHCRERPGA FPSEETLRGL GRRGFLQTLN ATLGCVLHRL ADLEQRLPKA QDLERSGLNI EDLEKLQMAR PNILGLRNNI YCMAQLLDNS DTAEPTKAGR GASQPPTPTP ASDAFQRKLE GCRFLHGYHR FMHSVGRVFS KWGESPNRSR RHSPHQALRK GVRRTRPSRK GKRLMTRGQL PR
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant Oncostatin M was lyophilized from a.2μm filtered concentrated (1mg/ml) PBS solution, pH7.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P05231 IL6_HUMAN
Interactor P42702 LIFR_HUMAN
Interactor P40189 IL6RB_HUMAN
Biological Process Growth-regulation
Molecular function Cytokine
Molecular function Mitogen