Human Neurotrophin-3 Recombinant
$70.00 – $4,700.00
|Source||Optimized DNA sequence encoding Human Neurotrophin-3 mature chain was expressed in Escherichia Coli.|
|Molecular weight||Human Neurotrophin-3 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately14 kDa. RecombinantNT-3 is a disulfide-linked homodimer protein consisting oftwoamino acid residue subunits, and migratesas an approximately27 kDa protein under non-reducing and as kDa under reducing conditions in SDS-PAGE.|
|Purity||>97%, as determined by SDS-PAGE and HPLC|
|Biological Activity||The ED(50) was determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of-50 ng/ml.|
|Protein Sequence||MSILFYVIFL AYLRGIQGNN MDQRSLPEDS LNSLIIKLIQ ADILKNKLSK QMVDVKENYQ STLPKAEAPR EPERGGPAKS AFQPVIAMDT ELLRQQRRYN SPRVLLSDST PLEPPPLYLM EDYVGSPVVA NRTSRRKRYA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK TGNSPVKQYF YETRCKEARP VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI RIDTSCVCAL SRKIGRT|
|Endotoxin||Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).|
|Presentation||Recombinant NT-3was lyophilized from a.2 μm filtered PBS solution pH7.5.|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.|
|Usage||This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|
Secretion of NT-3 from NT-3-PLGA carriers loaded with three different SF solutions groups for at least 4 weeks.
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