Human NEPH1 Recombinant

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Human NEPH1 Recombinant


SKU: RKQ96J84 Categories: , Tags: , , ,

accession Q96J84

Source Optimized DNA sequence encoding extracellular domain of NEPH1 (GLN17 - ALA499 ) including a C-terminal His tag was expressed in HEK293 cells.
Molecular weight Recombinant human NEPH1 is a protein consisting of 491 amino acid residue subunits,due to glycosylation migrates as an approximately 70kDa band protein on reduced SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Biological activity The activity was tested by the ability of immobilized recombinant human NEPH1 to bind mouse MS-1 endothelial cells, after 90 min 75% of cells will adhere.
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human NEPH1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Recombinant NEPH1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.