Human NEPH1 Recombinant
$160.00 – $1,950.00
| accession | Q96J84 |
| Source | Optimized DNA sequence encoding extracellular domain of NEPH1 (GLN17 - ALA499 ) including a C-terminal His tag was expressed in HEK293 cells. |
| Molecular weight | Recombinant human NEPH1 is a protein consisting of 491 amino acid residue subunits,due to glycosylation migrates as an approximately 70kDa band protein on reduced SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological activity | The activity was tested by the ability of immobilized recombinant human NEPH1 to bind mouse MS-1 endothelial cells, after 90 min 75% of cells will adhere. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Human NEPH1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | Recombinant NEPH1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |