Human MIP-3 beta (CCL19) Recombinant
Category: Recombinant Mouse Cytokines$70.00 – $2,700.00
Description
Accession
Q99731
Source
Optimized DNA sequence encoding Human MIP-3 (CCL19) mature chain was expressed in Escherichia Coli.
Molecular weight
Human CCL19, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately10kDa. Recombinant MIP-3b(CCL19) is a monomeric protein consisting of amino acid residue subunits and migrates as an approximately11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human dendritic cells using a concentration range of.0-50.0 ng/ml.
Protein Sequence
GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant CCL19 was lyophilized from a 0.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P32248
Interactor
Biological Process
Biological Process
Molecular function
Methods
In Vitro Migration assay
- X-displacement of WT and Fcgr2b−/− BMDCs incubated with ovalbumin or IgG-opsonised ovalbumin for 24 hours prior to incorporation within a collagen matrix and subsequent exposure to a CCL19 gradient.
