Human MIP-3 beta (CCL19) Recombinant

////Human MIP-3 beta (CCL19) Recombinant

Human MIP-3 beta (CCL19) Recombinant

$70.00$2,700.00


accession Q99731


Source Optimized DNA sequence encoding Human MIP-3 (CCL19) mature chain was expressed in Escherichia Coli.
Molecular weight Human CCL19, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately10kDa. Recombinant MIP-3b(CCL19) is a monomeric protein consisting of amino acid residue subunits and migrates as an approximately11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity >98%, as determined by SDS-PAGE and HPLC
Biological Activity Determined by its ability to chemoattract human dendritic cells using a concentration range of.0-50.0 ng/ml.

Protein Sequence GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation Recombinant CCL19 was lyophilized from a.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P32248
Interactor O43927 CXL13_HUMAN
Biological Process Chemotaxis
Biological Process Inflammatory-response
Molecular function Cytokine

Methods

In Vitro Migration assay

CCL19
  • X-displacement of WT and Fcgr2b−/− BMDCs incubated with ovalbumin or IgG-opsonised ovalbumin for 24 hours prior to incorporation within a collagen matrix and subsequent exposure to a CCL19 gradient.